FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2240_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2240_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756425
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG77100.279710131783016No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG72270.26218743481139517No Hit
GATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTATG57790.2096556227722503No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC55500.20134776023291037No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA54350.19717568952538161No Hit
AGATATTCCCTGAAACCATTGATATGCAAAGGCTCTTAAGAATGTACTAT52770.19144362716199426No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA51950.18846875935314764No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT51210.18578412255004217No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT46140.16739073256119794No Hit
CTCCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAG44000.15962705315762266No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA42860.1554912613258115No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA42320.15353220203705886No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA42230.1532056921556001No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT40680.14758246641936565No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG40360.14642154239640112No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC39960.14497038736769546No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC39810.14442620423193087No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT39770.1442810887290603No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG37280.13524764867536757No Hit
GTCTGTTTCTTTAGAATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG35410.1284634989161686No Hit
TTAGTAGACAGCTGAACAGTCAGATGTCTGTTTCTTTAGAATTCAATCTT35330.12817326791042746No Hit
GAAGAAAGTTTATAGTGATACAGGCCTAACTCAAGAAACAAGAAACATCT34710.12592397761593369No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA34400.12479933246868678No Hit
GGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAAT33860.12284027317993415No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT33660.12211469566558132No Hit
GAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA32640.11841425034238189No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT32310.11721704744369972No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT31980.11601984454501756No Hit
GCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC31190.11315381336332386No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA31080.11275474573042982No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT30720.11144870620459471No Hit
CCACCTTAGGTCATATGTAAGGCCACAAGACAAGTCAATAAATTCCAGAA29980.10876406940148924No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA29590.10734919324850124No Hit
AGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATCCAAAAGA29100.1055715283383368No Hit
CAGATTCTCAGCGAGATTCAAAGAAAGAGGACTCATCATCAGAAATGAGA28740.1042654888125017No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA28630.10386642117960765No Hit
ATTTCATCTCTCTACTGGTCATTAGTCTGTTCAGATGTCTTTTTCTTTAG27650.10031109135927878No Hit
GTCTCAGCCAGATTCAAAGAAAGAGGACTCATCATCAGAAATGAGAGAGA27570.10002086035353765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCG651.5679689E-978.061145
ACATGGT29600.059.515075
GTTGACA30300.058.0177081
GTTACGT3200.054.481511
TTACGTG3500.051.8419152
AGTCCGC1800.048.32347145
GACATGG36550.047.609264
GGTCCTA46850.044.8681149
ACGTGTT4050.044.7561344
GCCGACC1801.8189894E-1244.296516145
TATTGGG15450.044.1579062
ATCTATG14850.044.0254631
GGTTATA9300.043.7414251
CTGGTTG13850.043.442736
CAGTGCA20550.043.0880742
CATGGTC41200.042.5801286
GACGACT2050.042.4419144
GTATTAT30350.042.3645361
TGGTCCT50500.041.6251688
ACTGGTT14650.041.5675165
Produced by FastQC (version 0.11.5)