FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2240_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2240_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608011
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC31860.19813297297095606No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG22970.14284728151735282No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG20030.12456382450120056No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA19540.12151658166517516No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG19320.12014843182042909No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC19150.11909122512221622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAT1500.053.159348145
GACGACT1650.048.332694
GCCGACC2150.047.2028145
GGTTATA3350.045.543471
GAATAAC12700.040.6168481
GTAGAAA7800.038.151182
GTTGACA15250.038.1128771
AGACGAC2109.094947E-1237.981593
GACGAAA2109.094947E-1237.970963145
GCGTTAA1354.979265E-737.5874189
ATCTATG7950.036.5548021
CCGACTA2201.6370905E-1136.249524
ACATGGT15600.036.2461365
AATCGGC800.002038359636.24501145
CGAAAGC3250.035.6973843
AGCGAAA3300.035.2255361
GTATTAT13700.035.0005571
TAACCTA3750.034.796295
GTACTAG1051.8314346E-434.596511
TATTATT12500.033.0966152
Produced by FastQC (version 0.11.5)