FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2239_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2239_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683660
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT27450.16303766793770713No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG19830.11777912405117423No Hit
ATTTCATTTATTTAAGTTCTGATGTGTTCCTTCTACTAACTTTGGACTTT18430.10946390601427841No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC18180.10797904565054703No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT17410.10340567573025433No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG16960.10073292707553781No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC855.3523756E-542.6344647
CGCTATA700.001051289441.4729922
CGCGCCA1453.4995544E-529.9902489
ACGCGCC1504.4102373E-528.9905768
CCGACTA4650.028.068734
ATAGTCG1555.5148266E-528.0553958
GTTGACA15350.027.9145951
ACATGGT15350.027.3974385
GCCGACC1658.4405714E-526.355066145
GTATTAT24050.026.2719231
AGCGAAA4850.025.4562741
ATCTATG7450.025.3457241
GGTTATA6900.025.2609561
GATATAT9300.024.9893341
GTTATGT9150.024.6052761
TATTGGG14700.024.192582
GAACCGT2751.9764775E-723.719569
ACTCCGA4900.023.7143691
GCGAAAG4900.023.6988532
GTGATCG1851.8354297E-423.505879
Produced by FastQC (version 0.11.5)