FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2239_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2239_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051967
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14670.13945304367912684No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12120.11521273956312317No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT11880.1129312991757346No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA11310.10751287825568673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTC1703.8198777E-1142.6464165
GTTACGT1609.895302E-1040.8680571
GACGACT1451.9274012E-840.0012974
TTACGTG1651.3515091E-939.5881422
CGACTCC1859.640644E-1139.183016
TATACTG3950.038.5437245
GCGAAAG2650.035.6044272
AGCGAAA2501.8189894E-1234.874081
GTTGACA10450.032.6770671
ACATGGT11000.032.2949685
ACTCCGA3000.031.4835411
CGAAAGC3050.030.9158693
GTTATGT6350.029.7482281
AGACGAC2202.2542736E-829.6727493
CTATACT2451.9717845E-929.5927964
GTATTAT11300.029.57611
CCGAGGT3201.8189894E-1229.454084
CTAATAC2054.0232408E-728.3057123
GGTGCGA1050.00771456427.6146958
TATACTA1855.8171936E-627.4320165
Produced by FastQC (version 0.11.5)