FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2239_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2239_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2263524
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6700.056.3383142
CGGACCG803.7371512E-545.3104635
CGAAAGC9300.040.551043
GTGATCG4300.038.7730459
AGCGAAA11500.033.4877431
GACAGTA13050.033.328157
GTCGTAG1352.110908E-532.2942621
TTAGACG1453.4907633E-530.0019654
GTATGGA17600.027.6611351
GGATAGC14800.027.43125
ACGCGAT1400.001000757825.8888328
CAGTATG15900.023.2511049
CATAGCG1902.1966695E-422.8911787
GTATAAT5001.8189894E-1221.7986261
CTGCCGC6050.021.566896145
TTTACGA2053.6378903E-421.245782
TATGGAT19950.021.1037882
TGGATAG19400.020.9292054
TTGGACA21200.020.8622154
TATACTG15050.020.7133545
Produced by FastQC (version 0.11.5)