FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2239_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2239_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814822
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG29460.1623299695507328No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC22010.12127911167045582No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5850.055.8132172
GGATAGC10850.045.4322785
CGAAAGC7850.044.3466073
AGCGAAA7600.043.0065571
GTATGGA16250.036.6518821
GCACCGT6050.033.5478026
GATAGCA14750.033.4178356
AGGTACT10400.032.766774
CAGGTAC11350.030.6714423
GTCGTTT3201.8189894E-1229.4479318
GGTCGTT3450.029.4151047
GGTACTG12100.028.7568445
TGGATAG17350.028.4170234
GTGATCG4350.028.3283379
GCAGGTA12200.027.9523582
TATGGAT20700.026.2888362
GTCCTAT8950.025.9694481
GTATAAG10100.025.8890971
TTTGGAC12100.025.773413
TATACTG17200.025.7090935
Produced by FastQC (version 0.11.5)