FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2239_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2239_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865219
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA178130.955008500342319No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC152370.8169013933484487No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG146490.7853769450128913No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATATT116850.6264679911581428No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT113190.6068456304594796No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA108490.581647516993983No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT98380.5274447665394788No Hit
GGATACACCATGGACACAGTCAACAGAACACAAAGGGGAATTCTTGAGGA87810.4707758177457982No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG86370.46305554468402904No Hit
TTTCTTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAG85800.45999960326374545No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA66220.3550253348266343No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG63740.3417293089980319No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATATTCGAGA59670.3199088149970593No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT58980.31620951748829496No Hit
GGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGA55870.29953587219516853No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG55530.297713029944473No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA54550.2924589552218801No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA52020.2788948643564107No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC51020.273533563619071No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG47940.2570207573480648No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG46820.2510161005222443No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC46050.24688789895449276No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG45460.24372473151946233No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA43210.23166180486044802No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG41300.22142172045212918No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG37510.2011023906576118No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA37450.2007807126133714No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT37030.19852896630368874No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT35990.19295321353685546No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC35250.18898585099122409No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA35200.1887177859543571No Hit
GGTTTAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA34710.18609074859306066No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA34560.1852865534824597No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG34300.18389261529075138No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT33610.180193317781987No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA33360.17885299259765208No Hit
CAGCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAA33230.17815602350179793No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC32170.17247304472021782No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC31410.16839845615983967No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG31180.16716535699025156No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT30560.16384135053310095No Hit
GTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTG29350.1573541766409199No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC29280.15697888558930614No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC29230.15671082055243915No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC28560.15311874905842157No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG28560.15311874905842157No Hit
ATATTATTCACTTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC28430.1524217799625674No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG27950.14984835560864435No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA27860.14936583854228377No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG27360.14668518817361392No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT27170.1456665410335194No Hit
CGAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCC26970.14459428088605145No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT26460.1418600175100082No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTGCCAGCGCAAAAT26130.1400907882666861No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC25890.1388040760897246No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA25440.13639149075792173No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG25320.13574813466944094No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT25190.1350511655735868No Hit
CCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGC25120.13467587452197302No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA25090.13451503549985283No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGCGAATCA25060.13435419647773264No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC24730.13258496723441054No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA24640.13210245016804997No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG24280.1301723819026077No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA24240.12995792987311408No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG24130.12936818679200673No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT24080.12910012175513974No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA23770.12743811852656445No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA23610.1265803104085901No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG23450.12572250229061574No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC23410.12550805026112216No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG23360.12523998522425517No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT23340.12513275920950837No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG23300.12491830718001479No Hit
GAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGA23060.12363159500305325No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG22390.12003952350903566No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA22380.11998591050166227No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCT22290.1195033934353017No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT22230.11918171539106132No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG21650.11607216096340428No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA21610.11585770893391072No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC21450.11499990081593636No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC21240.11387402766109501No Hit
TCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCA21150.11339151059473446No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA20910.11210479841777293No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT20870.11189034638827934No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT20810.11156866834403896No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA20490.10985305210809025No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC19960.10701156271730022No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG19960.10701156271730022No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC19850.10642181963619285No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT19790.10610014159195247No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTG19490.10449175137075056No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA19460.10433091234863037No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT19400.10400923430438999No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA19370.10384839528226981No Hit
TTATATGCCCATACGTCTTGTATTTGGTCATCAATCTTATTATTGATCAT19280.10336587821590923No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG19040.1020791660389477No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA18690.1002027107808788No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA20450.0120.69552
GGTACTG21600.0115.4589545
CAGGTAC22100.0112.5700153
GTACTGA24300.0104.110736
AAAGTGC27650.0101.194498
AGTAGAA22450.0100.6277541
TCTTAAA29500.094.412633
CTTAAAA32000.087.454774
GACTTAA259.393328E-486.98764145
CTCGGTT259.393328E-486.98764145
AGGTACT29450.086.656994
AAGTGCC32950.085.357379
TAAAAGT34100.082.6935356
TCTGACC900.080.54412145
AGAAACA30600.074.396834
GGTCAAA3450.073.69241
TAGAAAC30950.072.882063
GCGAAAG3800.072.594792
TAGTGGT300.001937096972.4897145
CTGGCCG300.001937096972.4897145
Produced by FastQC (version 0.11.5)