FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2239_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2239_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1544715
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT147570.9553218554879055No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC118020.7640244316912829No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATATT106920.6921665161534652No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT106500.6894475679979802No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA104190.6744933531428128No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG83640.5414591041065828No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA70860.45872539594682515No Hit
GGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGA68320.4422822332922254No Hit
GGATACACCATGGACACAGTCAACAGAACACAAAGGGGAATTCTTGAGGA66440.43011170345338784No Hit
TTTCTTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAG65900.4266159129677643No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATATTCGAGA60840.39385906138025456No Hit
GTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTG58640.37961695199438084No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG55380.35851273535894973No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA51380.3326179910209974No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG46700.3023211401455932No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC44290.28671955668197696No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG44140.2857485037693037No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG41910.27131218380089533No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG41750.2702763940273772No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA40640.26309060247359545No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA36050.2333763833457952No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT35490.22975111913848184No Hit
GAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGA34510.22340690677568353No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT34120.22088216920273318No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG33830.21900480023823166No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG33060.21402006195317586No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA32320.20922953425065466No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG32170.20825848133798142No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT31480.20379163793968466No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA31200.201979005836028No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG31070.20113742664504458No Hit
CGAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCC30680.19861268907209423No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC30240.19576426719491946No Hit
CCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGC29820.19304531903943445No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT29020.187866370171844No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT28920.1872190015633952No Hit
GTCTAGGGCCCGAATTGATGCACGGATTGACTTCGAGTCTGGAAGGATTA28590.18508268515551413No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT28400.18385268479946137No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC27860.18035689431383783No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC27860.18035689431383783No Hit
GTTCAAGCACTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGG27750.17964478884454413No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTGCCAGCGCAAAAT27440.17763794615835285No Hit
TCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGGCCATGGTGTC26330.17045215460457105No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG26170.169416364831053No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA25530.1652732057369806No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA25450.16475531085022158No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA24820.16067688861699408No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG24510.15867004593080278No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT23990.155303729166869No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA23600.1527789915939186No Hit
ATGTAGGACCATTCTCTTCCTCCCAACAGCAGGTCACAAGAAGGGTTGCC23500.1521316229854698No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC23230.150383727742658No Hit
ATATTATTCACTTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC23220.15031899088181314No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA23040.1491537273866053No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCT22890.14818267447393207No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA22600.14630530550943055No Hit
CCCATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCTAG22240.14397477851901483No Hit
ATGGAGGCCATGGTGTCTAGGGCCCGAATTGATGCACGGATTGACTTCGA21950.1420974095545133No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA21640.140090566868322No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC21480.1390547770948039No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA21440.1387958296514244No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG20960.1356884603308701No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG20940.1355589866091803No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20820.13478214427904178No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA20820.13478214427904178No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC20690.1339405650880583No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG20650.13368161764467879No Hit
TCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCA20600.1333579333404544No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG20590.13329319647960952No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTG19930.12902056366384737No Hit
GGAACAGGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCA19870.12863214249877808No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA19810.1282437213337088No Hit
GGTTTAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA19440.12584845748244822No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG19340.12520108887399942No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT18670.1208637191973924No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT18340.11872740278951134No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG18200.11782108673768299No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG17920.11600845463402634No Hit
CTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCA17750.11490792799966336No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT17750.11490792799966336No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA17660.11432529625205945No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT17150.11102371634897053No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC17100.11070003204474613No Hit
GAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCCC16910.10947003168869339No Hit
CCCTAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG16910.10947003168869339No Hit
GAACAGGATATTCGAGAAATTCTTCCCTAGCAGTTCATATCGGAGGCCAG16700.10811055761095088No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG16550.10713950469827768No Hit
GAAAAGACAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAA16550.10713950469827768No Hit
GTGCATGGAAACAATTCGGAACGGGACCTATAATAGGAGAAAGTATAGAG15970.1033847667692746No Hit
TCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTA15930.10312581932589507No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT15930.10312581932589507No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT15910.10299634560420531No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA15900.10293160874336044No Hit
GAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGA15880.10280213502167065No Hit
CTTCCCTAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGG15880.10280213502167065No Hit
ATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCT15850.10260792443913602No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT15810.1023489769957565No Hit
GCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGC15700.10163687152646281No Hit
ATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGG15620.10111897663970376No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG15530.10053634489209985No Hit
CTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCAT15500.10034213430956519No Hit
ATTATATACAAATGTTGCATCTGCAAGATCCATTGGACATGGCCCAGAAC15450.10001845000534079No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTA100.0070724105145.128952
CCGCTTA100.0070916126144.99744
TAGTCCG100.007095045144.97394145
GCAGGTA14850.0110.923482
AGTAGAA25850.0107.350891
GGTACTG16050.0103.437175
CAGGTAC16250.0101.304633
CTGCCGC4100.093.70266145
GTACTGA18000.092.22556
GACTTAA259.394429E-486.98436145
CGACGAA451.2345245E-680.541084145
AGAAACA35150.078.995744
AGGTACT21650.077.689154
TAGAAAC36300.076.315643
GCGAAAG3900.072.564482
AGCGTGC300.001937323172.48698145
AACTGCG300.001937323172.48698145
CGCCGTT300.001937323172.48698145
AAAGCAC300.001937323172.48698145
AGCGAAA3950.071.724971
Produced by FastQC (version 0.11.5)