FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2239_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2239_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1959426
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG31800.1622924264555028No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC22990.11733027937773613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG2350.043.2456742
TCGCCAT12550.041.0087145
GCGTTAA2401.5861588E-930.2030079
GCGGTAT3400.029.84926
TATTAGT18300.029.7503872
AGAGGTC17750.029.4032639
GAATAAC15350.029.346371
TAGAGGT17650.029.1591648
TACTGCG2553.0286174E-928.4299875
GCGAAAG3850.028.2821872
CTATTAG21850.028.2643221
TAGCGGT3900.027.8853914
GGTTATA9450.027.6784521
CGGCGTT1100.00968212726.3589867
GTATTAT20100.025.6645341
GTGATCG4051.8189894E-1225.0573089
TGCGTTA3001.7123966E-824.1624038
ATAGCGG4303.6379788E-1223.614353
AGCGGTA4303.6379788E-1223.60355
GGATAGC5550.023.5123655
Produced by FastQC (version 0.11.5)