FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2239_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2239_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716610
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG24690.14382999050454093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAT10950.037.07621145
GCGAAAG4950.035.190152
TATAGCG2850.033.106522
CTATTAG12900.032.1009861
TATTAGT15500.029.9683882
CTGCCGC5400.029.535908145
CGAAAGC6250.029.0056513
GTATTAT16650.027.4890351
GGTACTG6100.027.3350225
GTACTGA7350.026.6308616
TATACTG8150.025.7965625
TATACGC4001.8189894E-1225.3732939
AGCGAAA7000.024.9084321
GTCGACA4101.8189894E-1224.754436
TAGAGGT16600.024.0194688
AGTCGAC4403.6379788E-1223.0673035
TATACTA6000.022.9574585
AGAGGTC17250.022.6941249
GCGGTAT3905.0386006E-1022.3061926
ATACGAC2303.138989E-522.0695173
Produced by FastQC (version 0.11.5)