FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278205
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT36620.28649551519513694No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG22530.17626280604441386No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC21690.1696910902398285No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA17010.13307724504285307No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG16220.12689670279806448No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC16150.12634905981434905No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT14190.11101505627031658No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATTTATTGTGTTTATT13990.1094503620311296No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA13890.10866801491153609No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC13070.10225276853086947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1051.0913936E-955.3423351
TTACGTG1206.002665E-1154.4264142
CCAACCG553.219251E-452.727574
TACGTGT1402.7102942E-1046.6238253
TATAGCG1751.8189894E-1245.6145172
ACATGGT6500.041.2694665
GCGAAAG3100.039.7956542
CGCTATA1651.3533281E-939.5828442
TAACTAT5850.038.4913061
GTATAGG10950.037.14761
TATACTA7050.035.9931145
GTTGACA8050.034.288191
CGAAAGC3800.032.445823
ATAGGTG12100.032.366953
TAGGTGG12800.030.5861114
TATACGT950.00472765130.5264914
GCGGTAT2651.382432E-1030.0909776
GGATAGC2901.2732926E-1130.000175
ACCGTGT5600.029.7722978
AGCGAAA4200.029.4006161
Produced by FastQC (version 0.11.5)