FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424098
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT34000.23874761427935437No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA23070.1619972782771972No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG22030.15469441007571108No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC21010.14753198164733045No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG19700.13833317650892005No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT19390.13615636002578474No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC16050.1127029179171658No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA15840.11122830029955803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC805.88052E-754.368256145
ACGACGC450.00964469348.32734145
ACATGGT6700.040.035165
GACGACC1001.392761E-436.245506145
GGATAGC4300.035.405075
CTGCCGC3950.034.86909145
CGCTATA2603.6379788E-1233.4997372
TGCGTTA900.00363094832.2182278
TTACGTG1153.1416764E-431.5577222
ATCTATG4600.030.0009291
GTTGACA9450.029.9758641
GCGAAAG2701.6916601E-1029.591561
GGTACTG3201.8189894E-1229.451545
CGTGATC1255.1586656E-428.9964078
GCGTTAA1000.006078991628.9964059
TTTAAAC9850.027.9827793
GTACTGC2354.2995453E-827.7664154
TAACGTA1050.007711553527.6175635
GACAGTA5300.027.3550997
GTATAGG11950.027.351651