FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013845
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG7050.040.14722
CGAAAGC7350.038.481763
AGCGAAA7450.038.023781
GCGTTAA2401.6011727E-624.1636339
GCCGACC2953.8831058E-722.11587145
GTATTAT23900.021.27391
ACCGTTC2755.2206688E-621.0882638
GATAGCG3851.0542863E-820.711685145
CCGACTA3208.4795647E-720.3905964
CAATTCG1800.004311694320.136369
GCACCGT7050.019.538016
TATACAT15250.019.4917765
TACTGCG3602.6193957E-618.1249755
AGGCGCG2400.001061111618.1227267
GTTATAC5652.75395E-916.686783
TACGAGG4901.6670128E-716.2734687
ACCGTGT7601.8189894E-1216.215078
TAGTACC15900.015.9590984
GTTGACA15600.015.8307441
GACGGTC2750.002638516515.8161967
Produced by FastQC (version 0.11.5)