FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593111
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG1451.9093932E-840.0464822
ATAGTCG750.001485488838.6569258
GCGAAAG5700.036.928832
CTGCCGC5100.032.68784145
AGCGAAA6300.032.2870671
CGCTATA2257.8398443E-1032.2596662
TATACCG1751.2567254E-424.8555625
CCGACTA2451.911194E-623.6764224
CGAAAGC8650.023.4799823
GTGATCG3102.4267138E-823.3812059
TGATCCG2202.2342272E-523.0623689
GCGGTAT2603.2096614E-622.3048736
CCGCTAT3808.8457455E-921.0290761
CCGTACA1750.003635123120.7383582
ATACCGA2205.9105037E-419.7702276
GTCGGTA1850.00505552719.589659145
CCTATAC4553.416062E-919.130473
ACCGTAC2307.857825E-418.9511031
CTAATAC5005.3842086E-1018.8594573
GAATAAC10600.018.5041431
Produced by FastQC (version 0.11.5)