FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893274
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC553.217123E-452.735953
GCGACTA701.6965058E-551.7860264
GTTGACA14350.038.958241
ACTCCGA4150.038.4888651
ACGATAA1957.010385E-933.45644145
ACATGGT16800.032.796085
GTTACGT3350.032.509251
CGACGAA1652.3907578E-630.752886145
GATAGCG5300.028.722034145
AGTCGAA2207.4536E-726.3791262
CCGAGGT6350.026.264
TTACGTG4200.025.9080682
GTATTAT21700.025.7627091
CTGCCGC7200.025.169773145
GACATGG21650.024.780754
CGAGGTG7250.023.9988775
TCCGAGG7000.023.8253483
TATTATT23000.023.6551932
TGACATG23150.023.4919853
GTTATGT9600.023.445041
Produced by FastQC (version 0.11.5)