FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774266
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC26540.14958298248402438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATACG858.9197056E-751.1839944
GCGTTAT450.00956199748.433291
ATAATCG450.00962832348.3486183
CGATACA1803.2105163E-936.242046145
GCCGACC2054.049889E-728.286472145
GTTGACA14700.027.1819441
CTGCCGC9850.026.491648145
TATGCCG1100.009674611526.363055
GTATTAT12050.025.3219641
TAGTACC7200.025.1680899
GCTACTA4101.8189894E-1224.7506649
GAATAAC10900.023.994471
ATCTATG7800.022.3538251
ACTCCGA4900.022.2397731
ATACGAG3655.702532E-921.8457536
GTTCTGA15000.021.7949771
GGTTATA4057.858034E-1021.5259041
TCGTAAG1700.00308805921.3302733
ACATGGT18850.020.768775
CTTATAT6300.020.7571221
Produced by FastQC (version 0.11.5)