FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227451
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCC1050.069.035988
GACGCGC851.6916601E-1068.223797
CGCGCCA1450.054.9907269
CCGACTA2400.039.273814
GACGACT1406.6073153E-736.252754
TATACTG9400.033.1633535
GCGATTA1352.1444699E-532.2181145
ACGACTC1851.644803E-731.34995
GCGAAAG4450.030.9940262
AAGGTCG2351.2641976E-930.8496325
GGTCGTT1902.0803054E-730.521177
GTCGTTT1952.6119778E-729.7385758
GTTACGT1753.7150348E-629.0602191
AGCGAAA4850.028.461041
CGAAAGC4700.027.7725893
TAGATCG1050.00771086727.617775
GTACCGG2405.2801624E-827.1840256
GCACCGT5350.027.099346
GCCGACC1358.089803E-426.84842145
CTGCCGC4100.026.521145
Produced by FastQC (version 0.11.5)