FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634426
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6300.047.255111
CGAAAGC6600.046.1507573
GCGAAAG6600.045.071242
GGTCGTT4950.032.2174847
GACGCGC1153.1657008E-431.5171037
TATATCG1204.0619317E-430.2048175
AAGGTCG5000.028.9966245
ACGCGCC1753.7808713E-628.9957358
TATACTG12150.028.638645
CGCGCCA1804.7077447E-628.1902989
GCCGACC1907.167253E-626.706596145
TTATACT12900.026.4140784
GTCGTTT5750.025.2136828
AGGTCGT5800.024.9963236
ATAGTCG1801.5223798E-424.1631138
ATACCGA2101.5576254E-524.1631136
CCTTATA12500.023.7976172
GTTATAG3904.947651E-1022.3420051
ATACTGG14800.021.5508846
CTGCCGC6200.021.045292145
Produced by FastQC (version 0.11.5)