FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307487
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC400.00605465554.3673828
AGCGAAA4550.041.5005381
GGTGCGA800.002038244936.2449238
GACGACA1209.575289E-636.244923145
GCGAAAG5350.035.2705232
CCGACGC2900.034.995102145
GCACCGT7900.031.1993546
CGAAAGC6250.030.1730823
AGCACCG8750.028.168565
CCGACTA3351.8189894E-1228.1336174
ACCGTGT9700.026.9034488
TATACTG8250.026.3609525
GATAGCG2207.4980926E-726.359943145
CACCGTG11350.023.6310527
CTGCCGC5800.022.496851145
CCGTGTT11700.022.3045679
ATAGCAC13650.021.7860033
CTTAGAC4058.021743E-1021.49083
TAGCACC12250.021.3055824
ACATAGC12300.020.6659181
Produced by FastQC (version 0.11.5)