FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861207
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC1950.059.479992145
CGCCTAT450.00964329248.3274887
GCCGACC1652.910383E-1143.934082145
GACGACA1351.0268195E-842.957767145
GCGAAAG3500.033.2179221
GGTATAC1204.0041498E-430.276751
AGCGAAA3650.029.8621
CGAAAGC4150.027.9955642
CTGCCGC4650.023.384268145
GTATGAC2608.047165E-519.528213
TATAGAC3001.0904099E-519.3635982
GTCCTAT5901.2732926E-1118.473951
TATACTG3953.1253876E-718.352215
ATAATAG3302.5013314E-517.5838413
TATATAG2901.8428135E-417.5273952
GCATAAT4006.9849757E-616.3494451
TTATAGA4501.2205346E-616.1129064
GTTCTGA11750.016.0788881
GTATAGA9250.015.711181
TATACAT6107.623385E-915.4489515
Produced by FastQC (version 0.11.5)