FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121606
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCC5800.047.4909328
CGCGCCA5900.046.6860059
GACGCGC6050.039.538847
GCGAAAG8150.036.5136262
AAACGCG1251.2665931E-534.796646
AGCGAAA8650.034.4281961
AGACGCG6800.034.1143576
TGATCCG6000.033.8268789
CGAAAGC10250.029.0074563
CGAATAT1804.7102167E-628.189064145
TAAGACG10200.026.2989624
ATAAGAC14050.024.7750513
TATATAG11150.024.0644823
ACCGTGT8800.023.8874868
ATAGTCG2151.869775E-523.600158
GTCCTAT16900.022.349261
GTGATCG2603.2122898E-622.3034369
TACCGTC2303.148228E-522.0615277
AACGCGC2003.108138E-421.746367
TAGACAG18800.021.2090595
Produced by FastQC (version 0.11.5)