FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2238_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2238_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567259
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATATGCAAAAAACCCCGGCCTTATTGACCGGGGTTTTTCT16380.10451367642489212No Hit
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG15790.10074914229237159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCA5450.049.212879
GCGAAAG5900.045.5043532
ACGCGCC6200.043.25978
GACGCGC6000.041.0772327
CGAAAGC6750.039.7513283
AGACGCG6900.036.76996
AGCGAAA7650.036.072171
ATAAGAC9500.032.824533
TGATCCG5900.031.9444079
AAGACGC8450.030.8849565
TAAGACG9100.028.6816334
GCCGACC3351.8189894E-1228.13015145
GACGGAT4350.026.6627067
AGTGCGC1100.00968023426.359728
GTATTAA20550.024.3830131
GTCATAG8050.024.3372292
GATAGTC4403.6379788E-1223.0647567
ATACCGC12000.022.9549246
GATGCGC2552.7122078E-622.741728
ACCGTGT5450.022.6113198
Produced by FastQC (version 0.11.5)