FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112849
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATATTCAGAGTGCTAAATGAGAAAACTAACCACCCAAGAGTACTTTA22190.10502406939634587No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7750.064.685951
TATATCG2350.055.52916
GCGAAAG9500.052.72262
ATCGAAT2600.050.1873869
TATCGAA2750.047.449898
CGAAAGC11050.044.6321333
ACCGTAC1557.2577677E-1042.1864931
TAAGACG1354.9731716E-737.5942464
ATATCGA3550.036.7569587
GACGCGC800.002038108236.2464457
TACACCG1000.00607606928.9998995
GTCCATA6400.024.9749871
GTGTAGG12950.024.6857031
GCAGCTA8250.024.6036478
GTATAAG12350.024.1201211
TAGGTCG1550.001809188523.3870165
AGAACCG6700.022.7238035
AGCGATC1600.002177186522.6540288
CCGTAGA3253.906098E-822.3552341
GTATATA14200.022.0010151
Produced by FastQC (version 0.11.5)