FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561389
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7150.078.230211
GCGAAAG7250.077.104152
ATCGGAT502.0102222E-458.0021744
CGAAAGC12200.048.7516373
TCGGATA850.00274324234.115655
TATCGGA1000.00606202529.013173
CTGCCGC6050.028.754965145
GAGCGAA2104.90465E-727.6732711
ACCGCGT1050.007717985727.613018
GTATATA8000.025.4248181
TTATCGG1450.001217122725.0338152
GGGTCGA3001.7125785E-824.1613839
GAAAGCA25150.023.927344
GTAGACC1550.001808546123.3879764
GCGCGCA2202.233614E-523.0631399
CAACGTA2603.211273E-622.303528145
GACGCGC1302.7721217E-722.3028147
TAAGACG1800.004307199720.1396474
CTAGGAC7250.020.0090833
GTAAGAG16050.019.4324973
Produced by FastQC (version 0.11.5)