FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822332
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4650.076.523891
CGATATA1550.065.557662
ATACCGT1750.053.851266
TATACCG1850.050.9431765
TCGAGCG901.3251774E-648.328056145
TACCGTA2050.045.970597
GCGAAAG7800.045.59622
CGAAAGC8250.043.077183
GCGATAT2700.037.654611
GGACCGT1354.978647E-737.5884866
CGAGTAC850.002737405234.1307753
ACCGTAT3000.033.829648
GTCGAGT900.003599831432.275381
ACTAGCG3350.030.2951987
CTAGCGC1703.0160736E-629.8496828
TACCGTC1255.1538105E-429.0016084
AACCGCG2752.1464075E-1028.9968347
CGTAAGG3204.1836756E-1127.2181382
CGGTCAA2001.0669992E-525.3722299
GTACCGT1450.001224081325.0096233
Produced by FastQC (version 0.11.5)