FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878156
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG9300.057.7593542
AGCGAAA9250.057.338791
GCCGACC1700.055.431263145
CGAAAGC13700.039.174443
GTGTAAC6750.027.985751
ATTCGGA1050.007696848427.628823
GTGATCG3850.026.3589219
GTATAAG3501.2551027E-1024.9103931
GTTATAG7000.022.8345261
GTAACGC5750.022.7036823
GCCGAGT3004.5671914E-721.746118
TAACGCA6350.021.6930754
CGCTCTC11050.020.991722145
CGCTTAC2556.84802E-519.9445951
TGTAACG7050.019.5631162
TATACCA16250.019.1832285
TCTATAC14400.019.1387143
GTATATA14100.019.0655781
TCGCCAT4954.765752E-1019.036999145
GTCCTAT12300.018.9021951
Produced by FastQC (version 0.11.5)