FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d08_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d08_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869717
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC19260.10301024165689245No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA6450.057.4488641
GCGAAAG6900.053.6633572
GTGATCG2250.038.6586279
CGAAAGC10150.038.590243
GTCCTAT16750.026.8935321
TATACTG9150.025.3512765
TATAATA18450.023.6108512
ACGTCCG4951.8189894E-1221.9651329
TCCTATG16800.021.1759832
AGCCGAC4501.3278623E-1020.940097
AATACCG1750.003663178820.7110885
GACGGGT2505.9876307E-520.295788
CTACTAG2205.8211444E-419.8152481
GCTAGTA2307.9766224E-418.9091139
TATACCA25750.018.5796555
GTATTAC8650.018.478961
TAACGTC5509.822543E-1118.450717
GTGTAGG10350.018.2517261
ATACAGC20850.018.078266
TACAGCC18300.017.8241637
Produced by FastQC (version 0.11.5)