FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1613049
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4600.058.4408531
GCGAAAG6750.039.781862
CGACGAA900.00363255732.21557145
CGAAAGC8850.031.1428243
TAGTACT3351.8189894E-1228.1354964
GCCGACC3357.4578566E-1125.964787145
CCTATAC3652.1827873E-1023.8454783
GTATTAG2852.723864E-722.9440611
GAGCGAA3508.547977E-820.7589131
CTATACT5251.8189894E-1220.7151454
CTAAACG1750.0036588120.7151454
GGGTACT2455.1246418E-520.7100097
CTGCCGC11300.020.526733145
GTATATA5153.6379788E-1119.75121
GTTACAT9950.019.6815073
TATAGTA5204.3655746E-1119.5394292
GTATTGA5759.094947E-1218.9537891
TCTATAC14300.018.2592983
TATAATA12100.017.9938072
TATAACG5751.8371793E-1017.6512645
Produced by FastQC (version 0.11.5)