FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027534
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG450.00964651148.324074145
CTAAACG800.002035637936.253644
TAAGACG800.002035637936.253644
CTGCCGC5450.034.58053145
GCGAAAG3100.030.4359052
AGTACCG1050.007712096427.6164445
GACGACA1907.1663453E-626.705406145
CGAAAGC3950.025.707543
AGCGAAA3851.6370905E-1124.5295411
TAGTACC4201.8189894E-1224.1690944
CCGACGC3203.392779E-822.651907145
ATAATAG7750.022.461523
GTATAGG3604.7384674E-922.197141
GTATATA8950.021.9153061
CTTAGAC3757.725248E-921.276053
GTATAAT8950.020.291951
GTATAAC5052.7284841E-1120.139261
GTATTAG4702.3101165E-1020.0933481
GTATAAG4402.2082531E-919.8123231
AGTACTC4452.6102498E-919.5468168
Produced by FastQC (version 0.11.5)