FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1696922
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG5050.051.74342
CCGACGC4300.038.77622145
AGCGAAA6750.038.7322961
CGAAAGC7000.038.3344233
GTGATCG3100.030.400999
TATACTG8450.026.5956755
TAGTACC6650.025.0755624
CCTATAC5850.024.794733
CTATACT6650.023.985324
ACGTAGA2552.6816651E-622.7716052
CACGTAG2104.2667278E-420.7494451
GTACAGT5555.456968E-1219.5931536
CTGCCGC10400.019.517794145
TCTATAC17750.019.2036933
GTCCTAT10650.019.0933881
GTGTAGG9150.019.0486721
TCCTATA9150.019.0385552
GTAACGC1950.00682909918.5960463
GTATTAT15050.018.336721
TTATACT6052.0008883E-1117.9754734
Produced by FastQC (version 0.11.5)