FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323682
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC40060.1723988049999957No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG31970.13758336984148434No Hit
TTAGTAGACAGCTGAACAGTCAGATGTCTGTTTCTTTAGAATTCAATCTT27530.11847576389540393No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT26230.11288119458686688No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG25170.10831946884298281No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1600.049.831615145
GTTGACA22600.048.5496561
GGTCCTA29650.043.0249339
ATGGTCC30150.042.3123327
ACATGGT26250.041.4256135
CATGGTC27200.039.7079666
GCGAAAG4700.037.06572
GACATGG31400.034.4055944
TTGACAT32000.034.251992
TGGTCCT38250.033.3513578
CTGCCGC6200.032.733963145
TGACATG34050.032.5923043
TATACTG17550.028.9153165
AGCGCTA3102.910383E-1128.0625115
TATAATA22850.027.954732
GCGTTAA2456.461414E-826.6261679
GCGTTCC8000.026.34061
CGAAAGC6150.025.9470673
GTACCGT1400.001000903225.8882226
TATACTA7050.025.740072
Produced by FastQC (version 0.11.5)