FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750382
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC21130.12071650645401974No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG953.1419404E-853.419376145
GCGAAAG4050.046.579012
CTACGAC1000.006069835329.0057183
CGAAAGC6800.028.7924443
CTGCCGC7550.026.886576145
GAATAAC10600.026.0284181
GTATTAT14550.025.449381
GACGAAC2651.3779754E-724.62187145
AGCGAAA7550.024.0415841
TAGTACT6000.022.9589274
TATAATA11300.021.8309362
GTATTAG6500.021.2231711
TATACCA29900.019.882325
GTATATA10400.019.5476571
TCTATAC26800.019.4814553
GTATAAT6850.019.078841
GTCCTAT18450.018.889261
AGGCGTT1950.00684379418.5891635
CGTTGGT1950.006844922418.5886338
TCCTATG19500.018.2319622
Produced by FastQC (version 0.11.5)