FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031457
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC23590.1161235507323069No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1905.4442353E-934.33414145
AGCGAAA6250.031.3845791
GTGATCG2558.913048E-1131.2664879
GCGAAAG6650.030.5575962
CGAAAGC6900.029.431583
GTATAAG9300.025.778851
GTCCTAT18900.025.7540511
CTGCCGC7200.024.161064145
GACGAAC2701.6543163E-724.161062145
GTATAAT9000.022.6020621
TTGTGCG6600.021.9640629
GTGTAAG19450.020.170041
GAATAAC15450.019.7493741
TATACTG11800.019.660335
TCCTATG22200.019.6146742
TCTATAC20550.019.4113393
TATAAGA13350.019.0269682
TATACCA31200.018.356735
TATAATA13500.017.7403832
CTGGTCG2500.00139646317.3955389
Produced by FastQC (version 0.11.5)