FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2237_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2237_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479441
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCG604.9498986E-448.325325145
GCGAAAG5700.035.6647872
CGAAAGC6500.032.3550073
GACGAAC1601.880353E-631.713493145
AGCGAAA6900.029.4931641
GTATCGG1050.007714590527.6154046
CTGCCGC7750.024.31855145
GTATTAG5052.7284841E-1120.1487941
GTATATA6800.019.2387141
GAATAAC9650.018.8289091
TATACAT10450.018.7302195
GTATTGA6750.018.3044871
ATATAGG6451.8189894E-1218.0290431
GTATTAT11700.018.0145971
GTGTAGG7300.017.9209941
TAGGACT12150.017.9000844
TCCTATG12900.017.4473082
GAGCGAA3753.7752689E-617.44311
GTCCTAT13000.017.3312851
GCGTATG2550.001593248217.055996145
Produced by FastQC (version 0.11.5)