FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d12_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d12_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869006
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAATACAGTGCAGATAAAGGCAGGTATGAACATTGGTGCTGGCCATT8790.10115004959689576No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC554.056079E-665.922983
GACGCGC1258.913048E-1152.1899727
AGCGAAA3150.048.404821
TACGCCT450.00964603248.324055
GCGAAAG3450.044.172792
ACGCGCC2200.042.8326848
AGACGCG1752.4338078E-937.2785536
CGAAAGC4100.037.1419753
CGCGCCA2650.035.5592049
TTAGGTA1854.1836756E-935.267564
CCTAATA4750.035.1389582
TGATCCG1102.4386363E-432.948229
CTAATAC6050.032.362193
ATAAGAC3550.030.6402593
CTACGCC1606.830702E-527.1854084
GTATAAG2859.73705E-925.4762211
GCACCGT4400.024.7111636
AGCACCG4550.023.8965115
GATATAT3952.1827873E-1123.8960481
GTTATAC1902.1897191E-422.8995633
Produced by FastQC (version 0.11.5)