FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586011
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA5400.055.1405681
GCGAAAG5600.054.4234352
CCGTACT3000.031.444652
CGAAAGC9600.030.969623
TATACTG6200.030.4015275
TCCGTAC4100.026.5698181
CTAGGCG1100.00966400626.3688143
CTATACT4600.025.2191624
TAAACGC2101.5564881E-524.165325
CTGCCGC8200.023.869865145
CCGACTA1550.001809874223.3850549
CGTACTC4053.274181E-1123.2761753
TATACAT8450.022.3064445
GAGCGAA2704.3820983E-621.518271
ATATACG1700.00309232421.3250274
ACGCGCC4001.6350896E-819.9357598
GACGCGC2205.908934E-419.7717
GACGTTC2608.08655E-519.5175258
GTATAGG3751.7775164E-719.3664441
ATTATAC8250.019.337133
Produced by FastQC (version 0.11.5)