FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d10_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d10_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370147
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3800.036.2926252
AGCGAAA4100.033.6678241
ACCGTAC1153.124188E-431.5876641
TACACCG1907.1576014E-626.7107015
ACGCGCC1400.001000675125.8883028
CGAAAGC5750.025.2258433
GTATTAG3501.2551027E-1024.909131
GCCGACC2051.2924795E-524.751743145
TATACTG5600.023.3037245
GTGATCG1902.1963604E-422.8907079
ACGTCCG1600.00217778422.6522649
CAACCGG4151.0950316E-920.960167145
GTTACGT1750.003615375120.7576081
GATATAC4201.2641976E-920.721233
TAACCCG2155.057226E-420.2326895
CGCGCCA1800.00431226220.1353459
GATATAT9100.019.9592381
CGATACT1850.00504655519.5954384
GTTGACA10100.019.4217221
GATAGCG3051.2774435E-519.013048145
Produced by FastQC (version 0.11.5)