FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718325
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6700.041.1787532
CGAAAGC6600.040.6623043
AGCGAAA6700.040.1348721
AACCGCG950.004733269630.519677
TACGCCG1453.4949502E-529.9960865
GTTATAC4000.027.1997833
GTCTACG1100.00955940126.4278761
GCCGACC2859.929863E-925.433058145
GTTACGT1902.1581931E-422.9505251
TAGTACT4256.184564E-1122.1774484
TAATACT8750.019.8865934
ACCGCGG2205.9144007E-419.7684218
GTATATG8700.019.213371
GTCCTAT16000.018.6233941
GTATAAG4704.989488E-918.5557441
CCGACTA2851.6333227E-417.8078044
TACGCAG2050.00913585617.6806225
TAGTACC9200.017.3331819
TATACAG8100.017.0039545
TATAGTA4701.0183612E-716.9925752
Produced by FastQC (version 0.11.5)