FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424653
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA15420.10823688294623321No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA7000.070.555011
GCGAAAG7100.069.51242
CGAAAGC8600.057.339783
ACGCGCC2700.053.6981548
CGCGCCA2750.052.721829
AACGCGC701.69739E-551.780367
GACGCGC2500.043.4955027
TAAGACG2550.042.647144
CCGACGC3150.039.12294145
GCCGACC2900.037.496124145
AAACGCG1001.3923051E-436.2475246
TGATCCG3050.035.652059
AGACGCG3800.028.6164676
TATACTG13700.028.0465155
TACGAGC1050.00771342327.616192145
ACTGATC9800.026.62998
CTGCCGC7200.026.17785145
ATACCGC8800.024.7142246
GCAGGTA6250.024.3865812
GAGCGAA2401.574108E-624.210051
Produced by FastQC (version 0.11.5)