FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451507
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA20250.13951017804254476No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT17770.12242448710202568No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG15980.11009247630221555No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA15520.1069233562084096No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG6250.056.888932
AGCGAAA6800.052.327321
CGAAAGC7600.046.761093
AAGGTCG3700.045.059759
CCGACGC2950.044.229614145
GGATAGC3550.042.884325
TATAGCG951.0239186E-438.1907462
GACGCGC2550.036.9543727
ACGCGCC3200.033.978488
GACGACA2257.9489837E-1032.216637145
GTCTACG1000.00602746929.0471
CGCGCCA3800.028.613469
CTGCCGC8200.028.287775145
TATACTA3600.028.1924675
TATACCG1804.7044505E-628.1924675
TGATCCG4950.027.8234569
AGACGCG3453.6379788E-1227.3159856
TATACTG15250.027.096465
GTCATAG5500.026.3863322
CCCTTAT14050.026.3593791
Produced by FastQC (version 0.11.5)