FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561233
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC3700.052.89574145
AACGCGC604.949617E-448.326057
ACGCGCC2150.047.2006848
CGCGCCA2300.044.1223759
GACGCGC1807.2759576E-1140.2717137
GCGAAAG4350.038.3742642
TGATCCG1901.2914825E-1038.1509259
CTGCCGC7150.031.427824145
GTTACGT2151.780245E-830.4036661
AGCGAAA5350.029.8669431
GCCGACC2951.4551915E-1129.48614145
CGAAAGC5550.028.7509843
TCCTACG1907.0996957E-626.7390462
AGACGCG2756.7775545E-926.3613536
TAAGACG3201.2405508E-924.9237084
GACGACA2603.211162E-622.303617145
ACGTCCG2003.1079538E-421.7460259
AGTGCGT2053.6722416E-421.2156378
ACCGTGT4301.7062121E-920.2288638
TATACTG11500.020.1728125
Produced by FastQC (version 0.11.5)