FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514417
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC3450.063.031322145
GTCTACG604.893631E-448.437561
GACGCGC1652.910383E-1143.930927
CGCGCCA2050.042.430849
ACGCGCC2250.038.659218
CTGCCGC7350.031.55854145
GCGAAAG4200.031.1384321
GCCGACC2401.5861588E-930.202509145
GTACCGA1050.00771445527.6155476
TATACTG9500.027.4729215
AAGGTCG2701.6531158E-724.1620069
AGACGCG3052.0390871E-823.7674776
GTATTAG6200.023.4375291
ATATACG1550.001803307923.3995843
GTGATCG2802.3486427E-723.2990769
TTATACT10150.022.8587724
CGAAAGC5850.022.3321462
ATAAGAC6900.022.0769983
GTTACGT2653.7120353E-621.9339891
GAATAAC12450.021.5926461
Produced by FastQC (version 0.11.5)