FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d02_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d02_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1812093
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC24030.13260908794416182No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG23160.1278080098537989No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA21300.1175436360054368No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA20920.1154466133912553No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT18930.10446483706962059No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG18790.10369224979071162No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC951.9250056E-645.7782367
CGCGCCA3250.044.6044359
TGATCCG3200.040.771249
GATAGCG2500.040.590034145
ACGCGCC3650.039.7162788
GACGAAC2057.2759576E-1238.89289145
GCGAAAG5250.038.7073752
AGCGAAA5250.037.3652271
GTTGACA13450.037.2726251
AAACGCG1209.578818E-636.2441026
CCGACTA3300.035.1535534
GTACTGC5400.032.2240944
GCCGACC1801.2961755E-732.214314145
TAAATCG1402.7468075E-531.0732334
GACGCGC3300.030.7500237
CGAAAGC6950.029.2167823
ATCGATA1000.00608303428.992888
GGTCCTA23300.027.3752529
ACATGGT17800.027.2878995
GTTACGT3306.002665E-1126.4198571
Produced by FastQC (version 0.11.5)