FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2235_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2235_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582445
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG3500.045.5593459
ACGCGCC2900.037.4900558
GCGAAAG4550.033.502422
GCCGACC2603.6379788E-1233.452663145
CTGCCGC6750.032.213676145
GACGAAC2051.1425072E-831.82083145
GACGCGC2755.456968E-1231.6279727
AGCGAAA4950.030.8224621
CGAAAGC5450.029.282353
CGCGCCA3550.028.5839659
GTCATAG7050.024.7110042
CATAGTG7600.022.8958994
GTGATCG2704.457799E-621.4757849
GAATAAC11450.020.9392761
GTTACGT1800.004255923420.1813761
GTATTAT11250.019.3741191
GTACTGC3401.51522E-619.192154
GTGATCC11350.019.1579138
GTATTAC7300.018.909671
TCTATGA10750.018.906792
Produced by FastQC (version 0.11.5)