FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2234_d06_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2234_d06_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906344
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT22460.11781714108261677No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC20680.1084798965978858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA554.0627438E-665.9098364
CGAAGCG400.00605208354.3741845
TAATCGT701.6983025E-551.7767839
GTTACGT3800.045.8814621
TTACGTG4000.043.5473252
GCCGACC2404.3655746E-1133.22344145
GACGACT2455.4569682E-1132.5513844
ACGTGTT5650.030.7968064
TGACGCG950.004732348530.521051145
AGCGAAA5250.030.4419881
GAGCGAC1204.0336326E-430.24122
CGAAAGC5500.029.0132663
GTATATC5050.028.7705551
GCGAAAG5700.028.01292
CCGAGGT6250.026.6803024
TACGTGT6900.026.2801323
AGACGAC3357.4578566E-1125.9820293
TAGTCGG3551.546141E-1024.5028178
CATTCTA23300.024.2967912
GATATGT9950.024.093531
Produced by FastQC (version 0.11.5)