FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2234_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2234_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319012
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC14900.11296333922663326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT2400.051.4371261
TTACGTG2950.041.816982
ACCGTAC1105.2075284E-639.6093371
GCGAAAG3850.039.580782
AGCGAAA3800.038.219541
GCTACTA2300.037.8182539
CGAAAGC4500.033.8442883
AGTCCGC1301.6575274E-533.45461145
ACGTGTT4000.030.8154664
GGTGCGA950.00473262730.5199978
CTGCCGC5400.029.530922145
TATTGGG8100.027.7716982
CCGACTA2903.947207E-1027.5026474
CCGTACA1606.788369E-527.2117842
CGATGTT1100.00967887326.3601765
AGTCTAT3501.2732926E-1024.8972991
GATAGCG4201.8189894E-1224.161665145
GTATTAT15600.023.7402131
GTACTAG1550.001791885823.4248771
GCGTTAA1902.196762E-422.8899979
Produced by FastQC (version 0.11.5)