FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2234_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2234_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914651
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA212131.1079303747784846No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG138310.7223770807316843No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA136860.7148038989873351No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG132590.6925021844712169No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT123120.6430414733546741No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT117480.6135844078111363No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG103330.5396805997542111No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA101570.5304883239817596No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT97250.5079254652675605No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT91990.48045309562943844No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT80840.4222179394573737No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG75660.39516340053618126No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC71970.37589095871780287No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT70710.36931012492616144No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA67990.35510388055055464No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG66420.346903952730811No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG64340.33604035409064104No Hit
CCCTTACACCATGGACACAGTCAACAGAACACATCAATAGCAGTTCATAT62150.3246022382146929No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT60670.3168723699514951No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT57710.3014126334250994No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC56620.2957196899069334No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC55460.2896611445114541No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA54640.2853783796629255No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC53740.2806777840974674No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC52490.2741491791454421No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC51220.26751611651418455No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT49240.25717480627017664No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT48060.25101180319546484No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG46580.24328193493226702No Hit
CTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTG45540.23785013561218207No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA45500.23764122025371726No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG45400.23711893185755523No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT44600.23294062468825913No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA43220.2257330448212233No Hit
GAATAATATAGTCGACAAGATGAACAAGCAATATGAAATAATTGATCATG42540.2221814837273216No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG40670.21241469071909186No Hit
GGATAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG39130.20437144941819682No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC38950.20343133030510518No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG38360.20034982876774932No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA36960.1930377912214811No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT36620.19126201067453025No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG35690.1864047285902235No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG35490.18536015179789947No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA33220.1735042052050217No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA32710.17084053438459543No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT31940.1668189137341479No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC31570.16488644666834842No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA31350.163737412196792No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC30130.15736549376361542No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG30090.1571565784051506No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTGAGGATGAAC29700.15511965366011873No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG29200.15250821167930867No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT29170.15235152516046005No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA28680.14979231201926618No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC28650.14963562550041756No Hit
ATATTGAGGATGAACAAATGTACCAGAAGTGCTGCACTCTATTCGAGAAA27920.14582292020843485No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT27520.14373376662378679No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA27420.14321147822762476No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG27280.14248027447299794No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA27170.14190575723721974No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA27020.14112232464297672No Hit
GAACAGCAGTTCATATCGGAGGCCAGTTGGAATTTCCAGCATGATGGAGG27010.14107009580336052No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA26780.13986883249218787No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG25910.13532492344557834No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC25860.13506377924749732No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG25850.13501155040788113No Hit
TTGTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTG25660.1340192024551733No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT25630.1338625159363247No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT25600.13370582941747608No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT25420.13276571030438447No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA24960.13036318368203917No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG24770.12937083572933136No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC24760.12931860688971514No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC24720.12910969153125035No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT24590.12843071661623973No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA24520.12806511473892632No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT24390.1273861398239157No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG24250.12665493606928885No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG24040.12555813043734862No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC23920.12493138436195422No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA23770.12414795176771119No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT23660.12357343453193298No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT23610.12331229033385195No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT23480.12263331541884134No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG23440.12242440006037653No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC23420.12231994238114413No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA23260.1214842809472849No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA23170.12101422139073909No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC23160.1209619925511229No Hit
CAATATGATCAATAATAAGATTGATGACCAAATACAAGACGTATGGGCAT22930.11976072923995026No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA22930.11976072923995026No Hit
CCCTTACACCATGGACACAGTCAACAGAACACATCAATATTGAGGATGAA22810.11913398316455584No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG22680.11845500824954522No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT22200.11594802394796755No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG22140.11563465091027032No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA22010.11495567599525972No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA21670.11317989544830885No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA21610.11286652241061165No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA21200.11072513998634738No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT21080.11009839391095295No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG20980.10957610551479094No Hit
GAACACATCAATATTGAGGATGAACAAATGTACCAGAAGTGCTGCACTCT20660.10790478264707251No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA20630.10774809612822389No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA20560.10738249425091048No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG20190.10545002718511103No Hit
GTACTAGCAATTCTGCATTATATGCCCATACGTCTTGTATTTGGTCATCA20150.10524111182664622No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA20120.10508442530779762No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG20110.10503219646818142No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA20100.1049799676285652No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT20060.1047710522701004No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA19950.10419653503432218No Hit
GAATCCCGCCAGCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGG19810.10346533127969537No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC19460.10163732189312831No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT19430.1014806353742797No Hit
TCTCTATACTTTCTCCTATTATAGGTCCCGTTCCGAATTGTTTCCATGCA19230.10043605858195566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA15350.0112.0850752
GGTACTG16350.0104.640675
CAGGTAC16750.0102.189833
TTACACC17950.094.91944
AGTAGAA29700.094.702691
GTACTGA18950.091.424146
GGTCAAA12300.085.087391
AGCGAAA5750.084.686621
TACACTA608.3127816E-1084.577145
CCCTTAC20950.083.9535751
GCGAAAG5950.081.745842
CCGCCAC451.2342389E-680.545456145
CCTTACA21200.079.786222
TTTCGTT1650.074.68761145
TCTGACC1200.072.490906145
CGAAAGC6850.071.999393
AAATATA14650.071.752435
GTCAAAT14800.070.6331562
AGGTACT25050.070.331544
CTGATCC24800.068.689199
Produced by FastQC (version 0.11.5)