FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2233_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2233_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAACTTATGTCAAAAATTATATGTACTGTCTGTCTATTGACAAGGC12.941176470588235No Hit
GTTGTGATCTCAGGGTCTTGGGGTCAAGCCCCACATCGGGCTCCACAATC12.941176470588235No Hit
ATTTGAGACGTTTATTGTTTCTTGAGTTAGGCCTGTATAACTATAAAATA12.941176470588235No Hit
TATCTTTGCAGTCCTCAAAGTCCAATTTTTCCGGTGCCATATTCTCCCCG12.941176470588235No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT12.941176470588235No Hit
TTATTATACAATAAACTTTCAATTTATATAGCTCCCAGCTCCCGCCAGGC12.941176470588235No Hit
ATATTACTATTTGCTTTTTTTCATGAAATGTATGCGTTACTATTTTAGTT12.941176470588235No Hit
GTAAATAAACACAATAAATTGTTGTCCAATGCCCATATCCTGACTCCCTA12.941176470588235No Hit
AAGCCAGACTCAAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAG12.941176470588235No Hit
ATATAGCCATGGAACAGGAACAGGATATTCGAGAAATTCTTCCCTAGCAG12.941176470588235No Hit
GAGGATGATTTTTCCTATGTGATCTTCTTTATTAATGGGGGATAAATGAG12.941176470588235No Hit
GAATACTTCATAACACATTAGATTAAATCTGAAATCATTTTAATGAATTA12.941176470588235No Hit
AAGCGAGAGAGAGAGATCACAAGTAGGCAGAGAGGCAGGCAGAGGGAGAG12.941176470588235No Hit
GCGTAGAAGAATTCTATAATTAACATCAAAACAAACGACTATAAAATGTC12.941176470588235No Hit
GGGTGAGGCAGGCGTGGAGCCTTGGGGGCCTGTGGGGCCACCTGGAGAGA12.941176470588235No Hit
ATCAAGTAGAGGACTATTTGGAGCCATAGCTGGATTCATAGAAGGAGGTT12.941176470588235No Hit
ACCCTAGCCTCCGGGATTGAGGTGAGATGAGATGGCACAGAGGAGGAGGC12.941176470588235No Hit
CTTCCTTCTTAGAACAACTTTTTTTTTTTTTAAACTAGGCTCTAACTGCT12.941176470588235No Hit
ACCCAAAACTATCCACGACTGCTGGCCAACTGGCGCACCAACCTGCTGGG12.941176470588235No Hit
AGCAGGATGAGCGTCTGACTCTTGATTTCAGCTCAGGTTGTGATCTCAGG12.941176470588235No Hit
TTTCTGTTCCTGTGGGGGGAATAGGTCCAGGTCCACTCAGGAGGCTTATG12.941176470588235No Hit
ACATGTGCTTCAAGAGCCTGAGACCCCATCTCTCCATGTTGCCCCACAGG12.941176470588235No Hit
CTCTCTTTCTGTCTCAACAATAAATACATATGTCTTTAAAAATCTTATCC12.941176470588235No Hit
CTCGTCATACTAATTCAAACTCTGCATTCCCACAGAGGCAGAGACAGAGG12.941176470588235No Hit
GGCCTAAGAAAATTAGGAGGTGAGCCTTCCTTCCTCGAGTAATTGTGGCA12.941176470588235No Hit
CATGTGGACCAATGAAACAGGTTAGAGAACCCAGAAATAAAGTCACACAC12.941176470588235No Hit
GACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGCTTAAATA12.941176470588235No Hit
CATAATAACCATGTTAGTGCATCTTCTCTGGGAAATTTGATTCGCATATC12.941176470588235No Hit
AAACTGTGGACACGCTAACAGAAACCAATGTTCCTGTGACACATGCCAAA12.941176470588235No Hit
GAAACAGACATCTGACTGTTCAGCTGTCTACTAATGACCAGTAGAGAGCT12.941176470588235No Hit
GTCTGGTTCTAGCACGAACTTCATTTTTTTTAAATTTTAATATTCTACAT12.941176470588235No Hit
GGTATTGGTGCTGATTGCTTTTCTTAGGTTTGTTCAGATATGCTCTAGAT12.941176470588235No Hit
GTTAACGTTCAACACAGATACAAGTCAGTAAAAATCTTGGGCACCTAGTG12.941176470588235No Hit
AACTAGAAGATACAGCACAATTATGAAAAATAAACAGCAACCACCAAATG12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)