FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2233_d04_replicate2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2233_d04_replicate2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326314
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC143790.6181022854180476No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA80100.3443215318310426No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT78990.3395500349479907No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT73730.3169391578265015No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA71580.3076970692692388No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG69370.2981970619615409No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT65990.2836676390203558No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA63620.2734798483781639No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC62630.2692241889959825No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA57150.2456676097895641No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT48410.20809744514283113No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA47460.20401373159427316No Hit
GGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAGGGGTTGTCTG46210.19864042429353904No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA46100.19816757325107442No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA44370.19073091594685843No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG44360.19068792948845253No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG43970.1890114576106235No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA42420.1823485565577132No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCACAT39290.16889379507667496No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA38600.16592772944666973No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG38590.16588474298826383No Hit
CTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTG37420.1608553273547767No Hit
GTTTTCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA35510.15264491379925496No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA35400.15217206275679035No Hit
CCCAAATATCTGCAAACCAAATTCTACAATACGGGAAGAGGTATGATCCA34740.14933495650200274No Hit
CCTAAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTT34290.14740056587373845No Hit
AGTCTGTTCAGATGTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTAT34280.14735757941533256No Hit
GAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACCAAATTCTAC33720.1449503377446037No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT33160.1425430960738748No Hit
ATACCATAGGGAACTTTAATACCTCACACTTCTCTCAATGATCCCCCATC32930.14155440753053972No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC31970.13742770752357591No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACT31580.13575123564574687No Hit
CTTCCCTCAATGATCCCCCATCCAGACAGAAGATCACAAAGGAAACATCA30270.1301200095945775No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA29840.12827159188312498No Hit
GAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA29760.127927700215878No Hit
CCCAACTGGTTGTTCAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATT28650.1231562033328261No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT28490.12246841999833213No Hit
ACCTGTTACTTAGATGGACTTGACAGATACACACAGAACCCTCCATCCAA28460.1223394606231145No Hit
GTACCCTTTTCTTTTGGATTATCCAGCTTGTTGGGGTGTAATTGTTCATA28050.12057701582847372No Hit
GGTTATATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTATCCAGCTTG27360.11761095019846848No Hit
GAGGCTGATGTTTCCGTTGTGATCTTCTGTCTGGATGGGGGATCATTGAG26290.11301139914904007No Hit
TTCTACATCTGTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAA25780.11081908977034055No Hit
GCGTTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTATTC25730.11060415747831118No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG24940.10720822726424722No Hit
ATTCTAGGCTTCTGGAATTTATTGACTTGTCTTGTGGCCTTACATATGAC24930.10716524080584135No Hit
AAATTCTACAATACGGGAAGAGGTATGATCCAGTAAGATGTATTTCAGGG24610.1057896741368534No Hit
CCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTGGGA24510.10535980955279468No Hit
TTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCA24340.10462903975989483No Hit
TTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAG24110.10364035121655976No Hit
GGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTATCCAGCTTG23890.10269464913163055No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATATCATAGGGAACT23410.10063129912814865No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATA6750.081.786951
ACATGGT40900.070.370575
GTTGACA41000.070.159341
CAGTGCA26150.069.946572
GCTACTA8250.067.6586469
GCAGTGC29950.061.8468441
AGTGCAG29800.061.810563
GACATGG46850.061.284
TTGACAT47150.061.11482
GTGCAGC30100.060.9378624
GCGTTAA2350.058.610079
TGCGTTA2400.057.389038
TATATGT10300.057.013934
GTTATAT9600.056.705822
TGCAGCA22550.055.9405255
CATGGTC52100.054.9610756
ATCTATG20200.053.5807881
CAGCAAC23800.053.3025057
CAGCTAC10550.052.22137
GGTCCTA65150.052.1849069
Produced by FastQC (version 0.11.5)