FastQCFastQC Report
Tue 22 Sep 2020
HNG7HBGXG_n02_2233_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNG7HBGXG_n02_2233_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799974
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC74430.41350597286405244No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT38960.2164475709093576No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT31920.1773358948518145No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG29800.1655579469481226No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA28550.15861340219358724No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC23680.13155745582991754No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG23630.13127967403973612No Hit
CCCAAATATCTGCAAACCAAATTCTACAATACGGGAAGAGGTATGATCCA23060.12811296163166802No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA22490.1249462492235999No Hit
ATCTATGATGAACACAAACATAAAAATGCTCACCCAAATATCTGCAAACC22460.12477958014949105No Hit
GTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCATTAGTTTGAT21830.12127952959320523No Hit
GGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAGGGGTTGTCTG21290.11827948625924598No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA21260.11811281718513712No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG21250.11805726082710084No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA21160.11755725360477429No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT21110.11727947181459288No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA20510.11394609033241591No Hit
GAATAACAGATCAGCAAAGAGCTGGTTCTTTGAAAAGAACAAAGCTGACA19390.10772377823235224No Hit
GTCTTTTTCTTTAGGATTCAATCTTAGTAGGTTGTATGTTTCTGGAAATG18700.10389038952784872No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA18510.10283481872515937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA801.8189894E-1281.5509959
TGCGTTA1154.0017767E-1156.7311338
GGTTATA4300.054.079581
TAGCGTA1800.052.3580935
CAGTGCA10400.050.9530222
TACTGCG1501.0913936E-1148.3305475
TGACGCG604.948905E-448.327866145
ACATGGT23250.045.5242545
GCCGACC2550.045.485046145
GTTGACA23500.044.8385851
GCAGTGC13000.040.8066831
GAATAAC13750.040.166381
GCGTATG2350.040.1018457
CTGCCGC7100.039.819435145
GTTATAT5700.039.47912
TATATGT6500.039.0394634
GTTCTGA19400.038.9568141
GCTACTA4750.038.1525159
TCTAGCG2500.037.7187843
ATCTATG10800.037.680451
Produced by FastQC (version 0.11.5)